- 品名： 美國(guó)BD腦心浸出液培養(yǎng)基
- 品牌： 美國(guó)BD
- 型號(hào)： 237500
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
用途:用于培養(yǎng)鏈球菌，肺炎鏈球菌、腦膜炎球菌等苛養(yǎng)菌。
成分* 含量 g/L
牛腦浸粉7.7g
牛心浸粉 9.8g
月示蛋白胨 10.0
葡萄糖 2.0
氯化鈉 5.0
磷酸二鈉 2.5
pH 7.4±0.2
Bacto? Brain Heart Infusion Approximate Formula* Per Liter
Calf Brains, Infusion from 200 g 7.7 g
Beef Heart, Infusion from 250 g 9.8 g
Proteose Peptone 10.0 g Dextrose 2.0 g
Sodium Chloride 5.0 g
Disodium Phosphate 2.5 g
可根據(jù)操作標(biāo)準(zhǔn)自行調(diào)整配方
制備方法：
溶解37g培養(yǎng)基至1L蒸餾水中，混勻分裝至最終容器中，121℃高壓滅菌15分鐘。
Directions：Suspend 37g of the powder in 1 L of purified water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Autoclave at 121°C for 15 minutes. Test samples of the finished product for performance using stable, typical control cultures.
Bacto? Brain Heart Infusion Dehydrated Appearance: Light tan, free-flowing, homogeneous. Solution: 3.7% solution, soluble in purified water upon boiling. Solution is light to medium amber, clear. Prepared Appearance: Light to medium amber, clear. Reaction of 3.7% Solution at 25°C: pH 7.4 ± 0.2
BHI肉湯是一種營(yíng)養(yǎng)豐富的緩沖培養(yǎng)基，含有大腦和心臟組織以及胃蛋白酶，以提供蛋白質(zhì)和其他必要的營(yíng)養(yǎng)物質(zhì)來(lái)支持挑剔和不挑剔的微生物的生長(zhǎng)。在含有6.5% 氯化鈉的配方中，鹽作為一種差異和/或選擇性介質(zhì)，干擾不耐鹽生物的膜通透性和滲透動(dòng)力學(xué)平衡。濾絲富集法(羊血消化法)被納入一種管狀配方，用于培養(yǎng)流感嗜血桿菌等較為挑剔的微生物。
